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犬基质金属蛋白酶1(MMP1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10057
  • Price:Negotiable
  • Clicks:2235

Product Detail

Product Information

Product NameDog MMP1(Matrix Metalloproteinase 1) ELISA Kit
Alternative NamesCLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase

Assay Parameters

SpeciesDog
Assay TypeSandwich
Sensitivity0.121 ng/mL
Detection Range0.32-20 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog MMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog MMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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