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犬胸腺激活调节趋化因子(TARC)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10055
  • Price:Negotiable
  • Clicks:2097

Product Detail

Product Information

Product NameDog TARC(ThymusActivation Regulated Chemokine) ELISA Kit
Alternative NamesCCL17, ABCD-2, SCYA17, A-152E5.3, Small Inducible Cytokine Subfamily A(Cys-Cys) Member 17

Assay Parameters

SpeciesDog
Assay TypeSandwich
Sensitivity16.59 pg/mL
Detection Range31.25-2000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog TARC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TARC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog TARC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog TARC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ95N01

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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