转化生长因子β1(TGF-b1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10034
- Price：Negotiable
- Clicks：1685
Product Detail
Product Information
| Product Name | TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit |
|---|---|
| Alternative Names | Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1 |
Assay Parameters
| Species | General |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Activated serum, plasma |
| Assay Length | 3.5h |
Research Information
| Research Area | Cytokine;Tumor immunity;Infection immunity; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF-b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF-b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGF-b1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
|---|
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