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牛组织蛋白酶B(CTSB)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10030
  • Price:Negotiable
  • Clicks:2150

Product Detail

Product Information

Product NameCattle CTSB(Cathepsin B) ELISA Kit
Alternative NamesCTS-B; APPS; CPSB; APP secretase

Assay Parameters

SpeciesCattle
Assay TypeSandwich
Sensitivity0.125 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CTSB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CTSB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CTSB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CTSB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP07688

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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