大鼠细胞色素P450家族成员27A1(CYP27A1)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10011MS
- Price：Negotiable
- Clicks：1926
Product Detail
Product Information
| Product Name | Rat CYP27A1(Cytochrome P450 27A1) Microsample ELISA Kit |
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| Alternative Names | CP27; CTX; CYP27; Sterol 27-Hydroxylase,Cerebrotendinous Xanthomatosis; Vitamin D(3) 25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.061 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYP27A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYP27A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYP27A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYP27A1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P17178 |
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Standard Curve
| Standard Curve |  |
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