小鼠血管紧张素转化酶(ACE)酶联免疫吸附检测试剂盒
- Cat. No.：ELK1001
- Price：Negotiable
- Clicks：1914
Product Detail
Product Information
| Product Name | Mouse ACE(Angiotensin I Converting Enzyme) ELISA Kit |
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| Alternative Names | CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.146 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase;Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACE in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P09470 |
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Standard Curve
| Standard Curve |  |
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