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小鼠肿瘤蛋白p73(TP73)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0985
  • Price:Negotiable
  • Clicks:2383

Product Detail

Product Information

Product NameMouse TP73(Tumor Protein p73) ELISA Kit
Alternative NamesP73; CILD47

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.052 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Metabolic pathway;Tumor immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TP73. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TP73. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TP73, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TP73 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9JJP2

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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