小鼠肿瘤蛋白p73(TP73)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0985
- Price：Negotiable
- Clicks：2332
Product Detail
Product Information
| Product Name | Mouse TP73(Tumor Protein p73) ELISA Kit |
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| Alternative Names | P73; CILD47 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.052 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Metabolic pathway;Tumor immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TP73. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TP73. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TP73, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TP73 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q9JJP2 |
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Standard Curve
| Standard Curve |  |
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