| Product Name | EasyStep Human ALD(Aldosterone) ELISA Kit |
|---|---|
| Alternative Names | ALD |
| Species | Human |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 10.7 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | Extracted urine, serum, plasma |
| Assay Length | 1.5h |
| Research Area | Metabolic pathway;Endocrinology;Hormone metabolism; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ALD antigen, and the Human ALD standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human ALD to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human ALD in the samples is then determined by comparing the OD of the samples to the standard curve. |
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