小鼠视神经萎缩蛋白1(OPA1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0973
- Price：Negotiable
- Clicks：2106
Product Detail
Product Information
| Product Name | Mouse OPA1(Optic Atrophy 1) ELISA Kit |
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| Alternative Names | MGM1; NPG; NTG; largeG; Dynamin-Like 120 kDa Protein, Mitochondrial; Optic atrophy protein 1 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.059 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Apoptosis; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OPA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OPA1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P58281 |
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Standard Curve
| Standard Curve |  |
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