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一步法人免疫球蛋白G(IgG)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK095ES
  • Price:Negotiable
  • Clicks:2434

Product Detail

Product Information

Product NameEasyStep Human IgG(Immunoglobulin G) ELISA Kit
Alternative NamesImmunoglobulin G

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity1.913 ng/mL
Detection Range3.125-200 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaInfection immunity;Immune molecule;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG, and the Human IgG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IgG. After TMB substrate solution is added, only those wells that contain Human IgG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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