小鼠肥大细胞类胰蛋白酶(MCT)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK0943MS
- Price：Negotiable
- Clicks：2326
Product Detail
Product Information
| Product Name | Mouse MCT(Mast Cell Tryptase) Microsample ELISA Kit |
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| Alternative Names | TPSAB1; TPS1; TPS2; TPSB1; Mcp7; Mcp-7; Mcpt7; MMCP-7 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 30.9 pg/mL |
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| Detection Range | 78.13-5000 pg/mL |
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| Sample Type | serum, plasma, tissue homogenates |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal Transduction |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MCT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MCT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MCT in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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