一步法人甲胎蛋白(aFP)酶联免疫吸附检测试剂盒
- Cat. No.：ELK091ES
- Price：Negotiable
- Clicks：1597
Product Detail
Product Information
| Product Name | EasyStep Human aFP(Alpha-Fetoprotein) ELISA Kit |
|---|---|
| Alternative Names | A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein |
Assay Parameters
| Species | Human |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.175 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
Research Information
| Research Area | Tumor immunity;Hepatology; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP, and the Human aFP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human aFP. After TMB substrate solution is added, only those wells that contain Human aFP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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