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人蛋白酪氨酸磷酸酶受体N(PTPRN)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0912
  • Price:Negotiable
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Product Detail

Product Information

Product NameHuman PTPRN(Protein Tyrosine Phosphatase Receptor Type N) ELISA Kit
Alternative NamesPTPRN; IA2; ICA512; PTPRN; R-PTP-N; CA512; IA2; lA-2; IA-2; PTP; ICA 512; ICA3; ICA512; islet cell antigen 2; Islet cell antigen 512; lslet cell autoantigen 3; protein tyrosine phosphatase; receptor type, N; protein tyrosine phosphatase-like N; PTP IA-2; receptor-type tyrosine-protein phosphatase-like N; R-PTP-N

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.119 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPRN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ16849

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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