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人葡萄糖转运蛋白8(GLUT8)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0861
  • Price:Negotiable
  • Clicks:1737

Product Detail

Product Information

Product NameHuman GLUT8(Glucose Transporter 8) ELISA Kit
Alternative NamesSLC2A8; SLC2-A8; GLUT; Solute Carrier Family 2 Member 8,Facilitated Glucose Transporter; Glucose transporter type 8, erythrocyte/brain; HepG2 glucose transporter

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.12 ng/mL
Detection Range0.32-20 ng/mL
Sample Typetissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Metabolic pathway;Endocrinology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GLUT8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLUT8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GLUT8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLUT8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9NY64

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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