Your Position:Home>Products>ELISA Kits>一步法人细胞程序性死亡蛋白1配体1(PDL1)酶联免疫吸附检测试剂盒

一步法人细胞程序性死亡蛋白1配体1(PDL1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK084ES
  • Price:Negotiable
  • Clicks:2707

Product Detail

Product Information

Product NameEasyStep Human PDL1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit
Alternative NamesCD274; PDL1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1; PDCD1LG1; Programmed Cell Death Protein 1 Ligand 1

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity1.23 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaSignal transduction;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDL1, and the Human PDL1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PDL1. After TMB substrate solution is added, only those wells that contain Human PDL1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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