人丙二酸单酰辅酶A(Malonyl CoA)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0846
- Price：Negotiable
- Clicks：1918
Product Detail
Product Information
| Product Name | Human Malonyl CoA (Malonyl Coenzyme A) ELISA Kit |
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| Alternative Names | malonyl coenzyme A |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.3 ng/mL |
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| Detection Range | 0.63-40 ng/mL |
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| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Malonyl CoA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Malonyl CoA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Malonyl CoA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Malonyl CoA in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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