人S-腺苷蛋氨酸(SAM)酶联免疫吸附检测试剂盒

Product Name	Human SAM(S-Adenosyl Methionine) ELISA Kit
Alternative Names	SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride

Species	Human
Assay Type	Competitive Inhibition
Sensitivity	5.8 ng/mL
Detection Range	15.63-1000 ng/mL
Sample Type	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length	2h

Research Area	Enzyme & Kinase;
Test Principle	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P17707

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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