| Product Name | Rat Glu(Glutamic Acid) ELISA Kit |
|---|---|
| Alternative Names | Glutamate |
| Species | Rat |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 50.1 ng/mL |
| Detection Range | 156.25-10000 ng/mL |
| Sample Type | biological fluids |
| Assay Length | 2h |
| Research Area | Enzyme & Kinase;Endocrinology;Neuro science; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Glu. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Glu. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Glu in the samples is then determined by comparing the OD of the samples to the standard curve. |
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