一步法人胰岛素样生长因子结合蛋白1(IGFBP1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK079ES
- Price：Negotiable
- Clicks：2030
Product Detail
Product Information
| Product Name | EasyStep Human IGFBP1(Insulin Like Growth Factor Binding Protein 1) ELISA Kit |
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| Alternative Names | IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.54 ng/mL |
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| Detection Range | 3.13-200 ng/mL |
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| Sample Type | Serum, plasma |
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| Assay Length | 1.5h |
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Research Information
| Research Area | Tumor immunity;Endocrinology;Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP1, and the Human IGFBP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP1. After TMB substrate solution is added, only those wells that contain Human IGFBP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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