人磷酸化AKT(S473)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0792
- Price：Negotiable
- Clicks：2325
Product Detail
Product Information
| Product Name | Human Phospho AKT S473 (p-AKT) ELISA Kit |
|---|---|
| Alternative Names | p-AKT |
Assay Parameters
| Species | Human |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 52.5 pg/mL |
| Detection Range | 156.25-10000 pg/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Enzyme & Kinase; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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