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大鼠晚期糖基化终末产物(AGE)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0785
  • Price:Negotiable
  • Clicks:2038

Product Detail

Product Information

Product NameRat AGE(Advanced Glycation End Product) ELISA Kit
Alternative NamesAGEs; Advanced Glycation End Products

Assay Parameters

SpeciesRat
Assay TypeCompetitive Inhibition
Sensitivity38 ng/mL
Detection Range125-8000 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2h

Research Information

Research AreaMetabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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