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人Argonaute-2蛋白(AGO2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0757
  • Price:Negotiable
  • Clicks:2107

Product Detail

Product Information

Product NameHuman AGO2(Protein Argonaute-2) ELISA Kit
Alternative NamesCASC7; EIF2C2; LINC00980; PPD; Q10

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity12.9 pg/mL
Detection Range31.25-2000 pg/mL
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaTumor immunity;Endocrinology;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGO2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGO2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGO2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGO2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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