人胃泌素17(G-17)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0740
- Price：Negotiable
- Clicks：2575
Product Detail
Product Information
| Product Name | Human G-17(Gastrin-17) ELISA Kit |
|---|---|
| Alternative Names | Gastrin17; Gas17 |
Assay Parameters
| Species | Human |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 14.3 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human G-17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human G-17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human G-17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human G-17 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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