| Product Name | EasyStep Human TF(Tissue Factor) ELISA Kit |
|---|---|
| Alternative Names | CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III |
| Species | Human |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.12 pg/mL |
| Detection Range | 12.5-800 pg/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Hematology;Cardiovascular biology; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TF, and the Human TF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TF. After TMB substrate solution is added, only those wells that contain Human TF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TF in the samples is then determined by comparing the OD of the samples to the standard curve. |
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