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人Mac-2结合蛋白糖基化异构体(M2BPGi)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0734
  • Price:Negotiable
  • Clicks:1987

Product Detail

Product Information

Product NameHuman M2BPGi(Mac-2 Binding Protein Glycosylation Isomer) ELISA Kit
Alternative NamesM2BPGI

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity6.91 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M2BPGi. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M2BPGi. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M2BPGi, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M2BPGi in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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