大鼠褪黑素(MT)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0721
- Price：Negotiable
- Clicks：1981
Product Detail
Product Information
| Product Name | Rat MT(Melatonin) ELISA Kit |
|---|---|
| Alternative Names | N-Acetyl-5-Methoxytryptamine |
Assay Parameters
| Species | Rat |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.78 pg/mL |
| Detection Range | 7.82-500 pg/mL |
| Sample Type | cell lysates and other biological fluids |
| Assay Length | 2.5h |
Research Information
| Research Area | Endocrinology; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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