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鸡谷胱甘肽S转移酶(GSTs)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0710
  • Price:Negotiable
  • Clicks:2534

Product Detail

Product Information

Product NameChicken GSTs(Glutathione S Transferases) ELISA Kit
Alternative NamesGSTs; GST-ST; GST ST

Assay Parameters

SpeciesChicken
Assay TypeSandwich
Sensitivity0.63 ng/mL
Detection Range1.57-100 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Metabolic pathway;Tumor immunity;Hepatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken GSTs. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GSTs. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken GSTs, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GSTs in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO73888

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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