鸡细胞色素P450家族成员1A5(CYP1A5)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0707
- Price：Negotiable
- Clicks：2054
Product Detail
Product Information
| Product Name | Chicken CYP1A5(Cytochrome P450 1A5) ELISA Kit |
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| Alternative Names | CYPIA5; Cytochrome P450 TCDDAA |
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Assay Parameters
| Species | Chicken |
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| Assay Type | Sandwich |
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| Sensitivity | 0.32 ng/mL |
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| Detection Range | 0.78-50 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CYP1A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CYP1A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CYP1A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CYP1A5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P79761 |
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Standard Curve
| Standard Curve |  |
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