猪主要急性期蛋白(MAP)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0706
- Price：Negotiable
- Clicks：2439
Product Detail
Product Information
| Product Name | Pig MAP(Major Acute Phase Protein) ELISA Kit |
|---|---|
| Alternative Names | IHRP |
Assay Parameters
| Species | Pig |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.059 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Immune molecule; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MAP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
|---|
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