人可替宁(Cot)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0704
- Price：Negotiable
- Clicks：1632
Product Detail
Product Information
| Product Name | Human Cot(Cotinine) ELISA Kit |
|---|---|
| Alternative Names | Cotinine |
Assay Parameters
| Species | Human |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.6 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2.5h |
Research Information
| Research Area | Signal transduction; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Cot. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cot in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
|---|
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