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人原癌基因MAS(MAS1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0703
  • Price:Negotiable
  • Clicks:2594

Product Detail

Product Information

Product NameHuman MAS1(Proto-oncogene Mas) ELISA Kit
Alternative NamesMAS; MGRA

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.12 ng/mL
Detection Range0.32-20 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAS1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP04201

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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