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小鼠血管紧张素Ⅱ受体1(AGTR1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0682
  • Price:Negotiable
  • Clicks:1982

Product Detail

Product Information

Product NameMouse AGTR1(Angiotensin II Receptor 1) ELISA Kit
Alternative NamesAT1R; AG2S; AG2-S; AGTR1A; AGTR1B; AT1; AT1B; AT2R1; AT2-R1; AT2R1A; AT2R1B; HAT1R; AT1 Receptor; Angiotensin Receptor 1B

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity29.8 pg/mL
Detection Range78.13-5000 pg/mL
Sample TypeTissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGTR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGTR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGTR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGTR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP29754

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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