一步法人干扰素γ(IFNg)酶联免疫吸附检测试剂盒
- Cat. No.：ELK067ES
- Price：Negotiable
- Clicks：2199
Product Detail
Product Information
| Product Name | EasyStep Human IFNg(Interferon Gamma) ELISA Kit |
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| Alternative Names | IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 15 pg/mL |
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| Detection Range | 15.63-1000 pg/mL |
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| Sample Type | Serum, plasma, cell |
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| Assay Length | 1.5h |
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Research Information
| Research Area | Cytokine;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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