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一步法人转铁蛋白(TRF)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK066ES
  • Price:Negotiable
  • Clicks:2204

Product Detail

Product Information

Product NameEasyStep Human TRF(Transferrin) ELISA Kit
Alternative NamesTF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity11.9 ng/mL
Detection Range15.63-1000 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaTumor immunity;Infection immunity;Immune molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TRF. After TMB substrate solution is added, only those wells that contain Human TRF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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