大鼠可溶性可罗索α(sKLa)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0662
- Price：Negotiable
- Clicks：2077
Product Detail
Product Information
| Product Name | Rat sKLa(Soluble Klotho Alpha) ELISA Kit |
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| Alternative Names | Klotho alpha-like protein |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 14.6 pg/mL |
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| Detection Range | 31.25-2000 pg/mL |
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| Sample Type | Serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sKLa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sKLa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sKLa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sKLa in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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