大鼠白介素1受体Ⅰ(IL1R1)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK0640MS
- Price：Negotiable
- Clicks：2465
Product Detail
Product Information
| Product Name | Rat IL1R1(Interleukin 1 Receptor Type I) Microsample ELISA Kit |
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| Alternative Names | IL-1R1;CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 14.9 pg/mL |
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| Detection Range | 31.25-2000 pg/mL |
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| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;CD & Adhesion molecule;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1R1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1R1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1R1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q02955 |
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Standard Curve
| Standard Curve |  |
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