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大鼠白介素1受体Ⅰ(IL1R1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0640
  • Price:Negotiable
  • Clicks:2429

Product Detail

Product Information

Product NameRat IL1R1(Interleukin 1 Receptor Type I) ELISA Kit
Alternative NamesIL-1R1;CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity5.96 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1R1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1R1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1R1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ02955

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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