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犬雌激素(Estrogen)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0620
  • Price:Negotiable
  • Clicks:2588

Product Detail

Product Information

Product NameDog Estrogen ELISA Kit
Alternative NamesE ; Estrogen

Assay Parameters

SpeciesDog
Assay TypeCompetitive Inhibition
Sensitivity3.96 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma, other biological fluids
Assay Length2.5h

Research Information

Research AreaReproductive science;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog strogen. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog strogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog strogen in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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