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一步法人胃蛋白酶原I(PGI)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK061ES
  • Price:Negotiable
  • Clicks:2721

Product Detail

Product Information

Product NameEasyStep Human PGI(Pepsinogen I) ELISA Kit
Alternative NamesPg5; PGA5; PGA; PG-A; Pepsinogen 5 Group I; Pepsinogen-5; Pepsin A-5

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity18 pg/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaEnzyme & Kinase;Tumor immunity;Gastroenterology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGI, and the Human PGI standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PGI. After TMB substrate solution is added, only those wells that contain Human PGI and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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