一步法人胰岛素样生长因子结合蛋白3(IGFBP3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK059ES
- Price：Negotiable
- Clicks：2073
Product Detail
Product Information
| Product Name | EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit |
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| Alternative Names | BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.98 pg/mL |
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| Detection Range | 78.13-5000 pg/mL |
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| Sample Type | Serum, plasma |
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| Assay Length | 1.5h |
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Research Information
| Research Area | Metabolic pathway;Tumor immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3, and the Human IGFBP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP3. After TMB substrate solution is added, only those wells that contain Human IGFBP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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