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兔髓分化因子88(MyD88)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0585
  • Price:Negotiable
  • Clicks:2603

Product Detail

Product Information

Product NameRabbit MyD88(Myeloid Differentiation Factor 88) ELISA Kit
Alternative NamesMyeloid Differentiation Primary Response Gene 88

Assay Parameters

SpeciesRabbit
Assay TypeSandwich
Sensitivity20.4 pg/mL
Detection Range62.5-4000 pg/mL
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Tumor immunity;Pulmonology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MyD88. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MyD88. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MyD88, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MyD88 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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