兔核因子κB亚基p65(NFκB-p65)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0584
- Price：Negotiable
- Clicks：2148
Product Detail
Product Information
| Product Name | Rabbit NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit |
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| Alternative Names | NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A |
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Assay Parameters
| Species | Rabbit |
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| Assay Type | Sandwich |
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| Sensitivity | 17.5 pg/mL |
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| Detection Range | 46.88-3000 pg/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Apoptosis; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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