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兔胰岛素样生长因子1受体(IGF1R)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0583
  • Price:Negotiable
  • Clicks:2488

Product Detail

Product Information

Product NameRabbit IGF1R(Insulin Like Growth Factor 1 Receptor) ELISA Kit
Alternative NamesCD221; IGF1-R; IGFIR; JTK13

Assay Parameters

SpeciesRabbit
Assay TypeSandwich
Sensitivity46.5 pg/mL
Detection Range125-8000 pg/mL
Sample Typetissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Endocrinology;Cardiovascular biology;Hormone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IGF1R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IGF1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IGF1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IGF1R in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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