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兔白介素23A(IL23A)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0581
  • Price:Negotiable
  • Clicks:2109

Product Detail

Product Information

Product NameRabbit IL23A(Interleukin 23 Subunit Alpha) ELISA Kit
Alternative NamesIL-23;IL23A; P19; IL23P19; SGRF; Interleukin-23 subunit alpha

Assay Parameters

SpeciesRabbit
Assay TypeSandwich
Sensitivity0.074 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL23A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IL23A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IL23A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IL23A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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