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人不育α和TIR基序包含蛋白1(SARM1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0577
  • Price:Negotiable
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Product Detail

Product Information

Product NameHuman SARM1(Sterile Alpha And TIR Motif-Containing Protein 1) ELISA Kit
Alternative NamesNADP(+) hydrolase SARM1; hSARM1; NADP(+) hydrolase SARM1; Sterile alpha and Armadillo repeat protein; Sterile alpha and Armadillo repeat protein;Sterile alpha and Armadillo repeat protein; Sterile alpha and Armadillo repeat protein; Tir-1 homolog

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.072 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaNeuro science
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ6SZW1

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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