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小鼠异丁二酸(MMA)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0576
  • Price:Negotiable
  • Clicks:1566

Product Detail

Product Information

Product NameMouse MMA(Methylmalonic Acid) ELISA Kit
Alternative NamesMMA; Methylmalonic Acid

Assay Parameters

SpeciesMouse
Assay TypeCompetitive Inhibition
Sensitivity0.17 ng/mL
Detection Range0.32-20 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2.5h

Research Information

Research AreaNeuro science
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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