人Ⅴ型胶原α3(COL5a3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0572
- Price：Negotiable
- Clicks：2499
Product Detail
Product Information
| Product Name | Human COL5a3(Collagen Type V Alpha 3) ELISA Kit |
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| Alternative Names | Collagen alpha-3(V) chain |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.055 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5a3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5a3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL5a3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL5a3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P25940 |
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Standard Curve
| Standard Curve |  |
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