一步法大鼠25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒
- Cat. No.：ELK053ES
- Price：Negotiable
- Clicks：2253
Product Detail
Product Information
| Product Name | EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
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| Alternative Names | 25 Hydroxy Vitamin D; |
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Assay Parameters
| Species | Rat |
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| Assay Type | Competitive Inhibition |
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| Sensitivity | 7.7 ng/mL |
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| Detection Range | 78.13-5000 ng/mL |
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| Sample Type | Serum, plasma |
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| Assay Length | 1.5h |
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Research Information
| Research Area | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
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| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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