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猴S100钙结合蛋白A9(S100A9)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0539
  • Price:Negotiable
  • Clicks:2472

Product Detail

Product Information

Product NameSimian S100A9(S100 Calcium Binding Protein A9) ELISA Kit
Alternative NamesMRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

Assay Parameters

SpeciesSimian
Assay TypeSandwich
Sensitivity0.27 ng/mL
Detection Range0.78-50 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian S100A9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian S100A9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian S100A9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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